Behaviour of Termites: Breeding and Effects of Repellents

Behaviour of Termites: Breeding and Effects of Repellents Introduction and Review of Literature General biology of termite Termites are social insects that live in colonies, which, in turn, function because the complementary roles played by the different caste. In United States, subterranean termites, Coptotermes spp. and Reticulitermes spp. are the most destructive and cause substantial economic damage to buildings/structures (Su Scheffrahn 1990). A population of subterranean termites is comprised of distinct colonies. Colonies may occupy underground networks that link several foraging sites (Thorne Breish 2001). Termite foraging behavior The nutritional ecology of termites has played a significant role in shaping their evolution and ecology organization (Thorne, 1999). To understand colony structure and the organization of foraging termites, it has been necessary to map both nest and feeding sites, frequently using behavioral assays of aggression to ascertain the colony affinity of a given nest or group of workers occupying a food source (Long Thorne 2006). Also, dye indicators and mark release recapture studies have been developed to attempt to census colonies, determine colony boundaries, and plot the distribution of foragers and other spatial aspects of resource use (Su et al. 1984, Forschler Townsend 1996, Thorne et al. 1996, Abdul Hafiz et al. 2007). Studies of termite foraging range generally offer statistic description of colony nest and satellite sub nest distribution and sometimes reveal the temporal dynamics of foraging range expansion and territorially, similar to what has been described in ants. Colony structure and the spatial organization of foraging in termites are, nevertheless, poorly understood in comparison to other social insects. The cryptic nesting and foraging habits of subterranean termites have hindered our understanding of many important features of their population biology, especially the distinctness of colonies and the breeding system within colonies. Their cryptic foraging and nesting habits, many features of subterranean termite biology have been difficult to study by using the traditional methods of field investigation. The main obstacle has been a lack of practical methods for delineating large numbers of colonies (Long Thorne 2006). Termite control Chemical and physical barriers to prevent them from reaching wood construction are two basic methods that have been used to control termites. Currently, insecticides used for termite control can be categorized into three groups, organochlorine, cyclodiens, which are being phased out of use because of potential damage to the environment and human health; pyrethroids, which considered not effective because of their repellency and short residual life in soil; and new termiticides that are considered as non repellent and slow acting termiticide (Potter and Hillary 2003). Remedial control is extremely difficult with acutely toxic chemicals because termites avoid area with dead termites, preventing any further contact with the toxicant. For the Formosan subterranean termites, remedial control using slow-acting toxic baits is are more acceptable method than the use of acutely toxic insecticides. Sub lethal insecticide exposure may be expected to influence insect behavior because most insect icides attack the nervous system resulting in detection by insects, distruption of physiological processes, and behavioral resistance (Haynes 1988, Silverman and Bieman 1993). The purpose of applying insecticides to soil proximal to a structures foundation of a continuous insecticide barrier in soils under and around the structure (Kamble, 1991). This barrier may be created by using either pre or post construction techniques. Post construction insecticide application technique include void treatments, soils trenching, sub slab injections, and soil rodding application. Chemical and physical barriers to prevent them from reaching wood construction are two basic methods that have been used to control termites (Abdul Hafiz et al. 2007). Remedial control is extremely difficult with acutely toxic chemicals because termites avoid area with dead termites, preventing any further contact with the toxicant. Sub lethal insecticide exposure may be expected to influence insect behavior because most insecticides attack the nervous system resulting in detection by insects, disruptions of physiological processes, and behavioral resistance (Haynes Baker 1988, Silverman Bi eman 1993) Non repellent termiticide Termiticide that do not repel termites from penetrating into treated soil but rather successfully kill them were recently registered in the United States (Kard 2003) and other parts of the world. They have become popular alternatives to conventional repellent soil-poisoning agents (Shelton and Grace 2003). Nonrepellent termiticides have been shown to be effective for the elimination of termite infestations when applied around the exterior perimeter of the building (Potter Hillary 2001) New generations of termiticides which contain imidacloprid (Premise ®), fipronil (Termidor ®) and indoxacarb, chlorfenapyr (Phantom ®), Chlorantraniliprole are used as soil-applied or by direct injection in the colony, posed lethal effects to subterranean termite (Osbrink Lax 2003; Kamble Davis, 2005; Hu et al., 2005; Spomer et al.,2009). The advantages of these new termiticides are slow-acting and non-repellent. Thus, termites cannot detect these termiticides when applied in to the soil or when used on filter paper in the laboratory. The transfer effect of imidacloprid, fipronil and indoxacarb among termite workers was studied by researchers and results showed that the termites were able to transfer the termiticides from the treated termites (donors) to the untreated termites (recipients) (Kard 2003, Thorne and Berisch 2001, Vargo Parman 2004, Tomalski and Vargo 2005, Hu et al. 2005, Shelton Grace 2006; Spomer et al.,2009). Termite baiting Baiting for suppression or elimination of subterranean termites is not a new idea. The ultimate goal of termite baits is to eliminate termites from structure (Su 1994, Su et al. 2000). Recently, hexaflumuron baits have successfully been applied to control the field colonies of Coptotermes spp. and Reticulitermes spp. (Su 1994, Su et al. 2000). At the same time Nouviflumuron also have been widely used in termite baiting. In laboratory trials in which R. flavipes were fed radiolabeled noviflumuron or hexaflumuron, noviflumuron demonstrated significantly faster speed of action, greater potency, and nearly 4-fold slower clearance from termites compared with that of hexaflumuron (Sheets et al. 2000; Karr et al. 2004, Spomer Kamble, 2006). Primer pheromones (Wilson Bossert, 1963) are chemical messengers that can be passed among individuals, thereby triggering a physiological response in a recipient. In termites, JH is one such primer pheromone (Henderson 1996); it includes presoldiers differentiation from workers at excessively high titer. The differentiation of the soldier caste, a development end point, in respond to JH is indirect in maintaining immature features in all other non social insects (Henderson, 1996). In the last decade there has been a dramatic increase in the research on and the development of bait systems for termite management. The increasing availability of bait systems for the control of active termite infestations is already significantly affecting termite management practices in many part of the world. There is even discussion of using such system as standalone measures for the long- term protection of a structure (Su et al. 2001; Grace et al. 1996). Even though these important and remarkable trends, baiting is developing technology, and many more changes to existing bait technology can be expected (Potter 1997). The bait matrices containing hexaflumuron, a chitin synthesis inhibitor, were most effective in reducing or eliminating foraging populations of Coptotermes formasanus Shiraki and Reticulitermes flavipes Kollar (Su Scheffrahn, 1996, Su, 1994). Molecular genetic marker The application of molecular genetic markers to the field populations of subterranean termite provides a powerful way to discriminate among large numbers of colonies be identified and distinguished from each other. The application of molecular genetic marker to field populations of subterranean termites provides a powerful way to discriminate among large numbers of conspecific colonies in a population as well as to determine colony breeding structure (Husseneder et.al. 2003) Genetic markers provide the most powerful means for delineating the boundaries of colonies and for determining colony affiliation for groups of foraging workers (Thorne et al.1999, Vargo 2003a,b). Microsatellite markers, with their co-dominant nature and high variability, are especially useful for investigation of colony distinctness (Vargo 2003b). Genetic markers are also the most practical way to determine colony breeding structure (Thorne et al.1999, Ross 2001), and there have been a growing number of genetic studies of breeding systems in termites (Luykx 1993, Husseneder et al. 1997, 1999, Husseneder Grace 2001a, 2001b, Vargo et al. 2003), including some on Reticulitermes spp. (Cle ´ment 1986, Reilly 1987, Jenkins et al. 2002, Bulmer et al. 2001, Vargo 2003b). The most detailed studies to date of the eastern subterranean termite, R. flavipes, are those of Reilly (1987) using allozymes, Bulmer et al. (2001) using allozymes and mitochondrial DNA haplotype data, and Vargo (2003a,b) using microsatellites and mitochondrial DNA sequence data. These studies have revealed variation in colony social organization in R. flavipes from very highly inbred colonies in Tennessee (Reilly 1987), to a mixture of approximately one third simple families and two thirds inbred colonies headed by many neotenic reproductives in Massachusetts (Bu lmer et al. 2001), to three fourths simple families and one fourth inbred families with only a few neotenics in North Carolina (Vargo 2003b). The above studies were conducted in natural areas, and there are no comparable studies performed to date in urban areas around buildings. Colony social organization in R. flavipes may vary in response to local ecological conditions (Bulmer et al. 2001), raising the possibility that for a given geographic area, colonies in urban habitats may differ in their social organization from those in natural habitats. In a study of Reticulitermes Hesperus Banks in southern California, Haagsma and Rust (1995) found differences in colony size, foraging activity, and body weight between colonies in natural and urban habitats. In addition to providing a powerful way to determine colony distinctness and to infer colony breeding structure in subterranean termites, molecular genetic markers are useful for applied studies, such as tracking colonies over time after exposure to an insecticide treatment and determining whether termites that reappear after treatment are part of the originally treated colony or are from a neighboring untreated colony that has moved into the area (Husseneder et al. 2003). Such studies are critical in the evaluation of management practices that target septic colonies and aim to eliminate them or greatly suppress their populations. Genetic/breeding structure population Colony fusion For social organisms, genetic structure assumes additional significant not only for the evaluation of social behavior, but also for the evolution of multiple queen societies, reproductive skew, sex ratio conflict, conflict over growth versus reproduction. Colony fusion has long been a suspected mechanism generating unusual colony genetic structure in termites. Colony genetic structure, reported a lack of genetic differentiation between colonies of Reticulitermes grasseiacross some regions of France and Spain. Together with this genetic data, a lack of distinct nest mate discrimination in laboratory trials (Clement 1986) suggested that colony fusion was widespread in some populations. Although recent studies have failed to corroborate these earlier descriptions of widespread colony fusion in this species (DeHeer et al. 2005), Clements work raised considerable awareness about colony fusion for those working on other subterranean termites. In introduced populations of Coptotermes formos anus, inter-colony aggression is often found to be weak or variable (Husseneder and Grace 2001a, Cornelius Osbrink, 2003) and one mark-release-recapture study described patterns consistent with a colony fusion event (Su and Scheffrahn, 1988). Nevertheless, genetic evidence for colony fusion in this species has remained elusive in spite of the relatively large numbers of colonies which have been assayed (Vargo et al., 2003, 2006; Husseneder et al., 2005). For Reticulitermes flavipes many experimental results have suggested the possibility that colony boundaries were porous. Laboratory agonism studies (Grace 1996, Polizzi and Forschler 1998, 1999, Bulmer and Traniello 2002, Fisher and Gold, 2003) and field surveys of molecular diversity (Jenkins et al. 2002, Bulmer et al. 2001) both suggested that individuals originating from different colonies may not distinguish between nestmates and non-nestmates, and consequently share the same nests or tunnel systems. However, in contrast to other species of subterranean termites these findings in R. flavipes have been confirmed via more direct assessments of colony fusion (Fisher et al. 2004), including one molecular study that provided a time course of colony genotypes before and after merger (DeHeer and Vargo 2004). Such fused colonies also appear to occur in other groups of termites. The relatedness hypothesis indicates that fused colonies are preferentially formed between groups of related termites because any costs associated with fusion are reduced when relatedness remains high in these colonies. This argument necessarily assumes that such colonies experience some general benefits to increasing their size (Costa and Ross, 2003). The genetic diversity hypothesis posits that increased genetic diversity provides sufficient group-level benefits to offset the costs of decreased relatedness within colonies. The specific benefits of increased genetic diversity could include the same types of benefits hypothesized to occur elsewhere (Schmid-Hempel and Crozier, 1999), in addition to the potential benefit that could result from a reduction in inbreeding. Simple family extend family Subterranean termite colony such as Coptotermes formosanus, generally begin as simple families headed by two primary (alate derived) reproductives that pair after mating (Tamashiro et al. 1987, Raina et al. 2003). As time goes by, the primary king and/or queen will be replaced by varying numbers of neotenics (non alate derived reproductives) from within colony; where this replacement leads to extended families with different degrees of inbreeding depending on the number of reproductives and number of generation of inbreeding (Thorne et.al 1999, Bulmer et al. 2001, Vargo et al. 2006, Husseneder et al. 2007) Bioinformatics/ Computational Molecular Biology Biological database is a large, organized body of persistent data, usually associated with computerized software designed to update, query, and retrieve components of the data stored within the system. A simple database can be a single file containing many records, each of which includes the same set of information (Lewis et al. 1995). For example, a record associated with a nucleotide sequence database typically contains information such as contact name, the input sequence with a description of the type of molecule, the scientific name of the source organism from which it was isolated, and often, literature citations associated with the sequence. The term of Bioinformatics meaning the application of information technology to the field of molecular biology. Bioinformatics currently contains the creation and advancement of databases, algorithms, computational and statistical techniques, and theory to solve formal and practical problems arising from the management and analysis of biological data (Hogeweg1980). Over the past few decades rapid developments in genomic and other molecular research technologies and developments in information technologies have combined to produce a tremendous amount of information related to molecular biology. It is the name given to these mathematical and computing approaches used to glean understanding of biological processes. Common activities in bioinformatics include mapping and analyzing DNA and protein sequences, aligning different DNA and protein sequences to compare them and creating and viewing 3-D models of protein structures (Huang 2004) Bioinformatics focus on developing and applying computationally intensive techniques (data mining, machine learning algorithms, and visualization) to achieve this goal. Major research efforts in the field include sequence alignment, gene finding, genome assembly, protein structure alignment, protein structure prediction, prediction of gene expression and protein-protein interactions, genome-wide association studies and the modeling of evolution (Huang 2004). In the application of molecular ecology studies of termites, mitochondrial genes were used for taxonomy purpose in termites (Cameron Whitting, 2007). Thus, the whole mitochondrial genome can be used to predict useful regions for further investigation in the multiple alignments methods application. Multiple alignments are the key starting point for prediction of protein secondary structure, residue accessibility, and the identification of residues important for specificity. Multiple alignments can provide the basis for the most sensitive sequence searching algorithms (Barton Sternberg, 1990). Furthermore, methods in multiple alignments affective analysis of a well-constructed multiple alignments can provide important clues about which residues in the protein are important for stabilizing the second and tertiary structure of the protein (Altschul et al. 1997) ClustalW will calculates the best match for the selected sequences, and lines them up so that the identities, similarities and differences can be seen and will provide a better quality for sequences (Thomson et al. 1994). Meanwhile, T-Cofee application is better to overcome with some of the problems that standard hierarchical method s have in aligning sequences of very different length or that share only local region similarity (Notredame et al. 2000). The program works by building a library of pairwise alignments for the sequence interest. Furthermore, T coffee has been adapted to include structural alignment and alignments from threading algorithms since it can work from pairwise alignments that originate from any source (Notredame et al. 2000). In addition, the program PSI-BLAST searches database with a single sequence, any high-scoring sequences that are found are built into a multiple alignment, and this multiple alignment is then used to derive a search profile for subsequent s earch of the database. This process is repeated until no new sequences are added to profile, or a specific number of iteration have been performed (Jones 1999). Research Objectives To test non-repellent termiticide model assay for their ability to induce detectable molecular changes in worker termites e. g., hemolymph proteins their corresponding genes. To infer the colony social organization of R. flavipes in urban habitat based on colony and population genetic structure and to compare this social organization in nearby natural habitat, To track the foraging locations of a large number of Reticulitermes colonies from natural forest (Wilderness Park) over the course of three field seasons using previously developed microsatellites by Vargo (2000). To analyze colony and population genetic structure/breeding system in natural and urban regions in order to determine the prevalence of neotenic reproduction and improve our understanding of the factors facilitating the spread of subterranean termites to new urban areas To determine the termite colony either a simple colony or extended colony using molecular ecology methods and also to determine if the colony undergo any kind of colony fusion To delineate the genetic structure of swarm aggregations of alates in order to infer potential mechanisms leading to inbreeding avoidance that do not require kin recognition. To utilize bioinformatic/molecular computational biology application tools to analyzing data at molecular level using BLAST, Clustal X, TCofee for sequencing analysis, pairwise/multiple alignment to study colony breeding structure of R.flavipes MATERIALS AND METHODS Termite Collection Sampling Reticulitermes flavipes, the Eastern subterranean termite, will be collected from locations within Wilderness Park Recreational Area, Lincoln; NE. Twenty workers will be sampled in each collection point located every 20 m along two intersection transects. The termites will be maintained on moistened corrugated cardboard and only 3-5th instar worker termites will be used for the study. Detection of fused colonies In order to detect colonies that had undergone fusion, collections of termites from large numbers of established colonies of R. flavipes will be collected between May 2009 and October 2011. At each location, minimum of 20 adult workers in 95%ethanol from multiple feeding sites, and mapped the locations of these feeding sites relative to one another using compass and measuring tape (DeHeer and Vargo, 2004). Samples will be stored in alcohol at 480C until DNAisolations could be performed. Microsatellite genotyping (Fusion Inbreeding) Using the Puregene DNA purification kit (Gentra systems, Minnepolis MN, www.gentra.com), genomic DNA will be isolated from each of 20 whole worker bodies from each of the feeding sites. At the location, minimum of 20 adult workers in 95% ethanol from multiple feeding sites will be collected, and mapped the locations of these feeding sites, and the locations of these feeding sites will be mapped relative to one another using compass and measuring tape. Each genotype individual termites at six different microsatellite loci : Rf1-3, Rf21-1 and Rf24-2 from Vargo (2000) and Rs15, Rs76, and Rs78 from Dronnet et al. (2004). PCR reactions will be setup in 96oC. Well plates in 5ul reaction mixtures containing 1X PCR buffer (Eppendorf), 2mM MgCl2, 0.2 mM of each dNTP, 0.2 U Taq polymerase (Eppendorf), 0.4 pmol of forward primer. Forward primers will be labeled with IRD 700 fluorescent dye (LI-COR), and samples were run on Li-Cor 4200L automated sequencer. Gel image will scored manually on Adobe Photoshop 7.0 by comparison to a size ladder. Colony affiliations To determine colony affiliation of the collection points, methods of other studies will be followed. Testing all pairs of collection points within each species for genotypic differentiation by means of a permutation test by using the program FSTAT (Goudet 2001). Pairs of collection points that will be significantly differentiated will be grouped into the same colony Morphology Scanning electron microscopic pictures (coronal and profile views) will made of the heads for each species) and will be studied using a binocular microscope to evaluate the profile of the post clypeus. Colour of the tibia of reproductives will be noted because this feature is an informative morphological indicator in association with the postclypeus profile. Genetic Data Analysis/ Simple or Extend Colony (Breeding Structure) The collection points will be analyzed to determine belonged to the same colony. The genotypic frequencies between all pairs of collection points by means of a log-likehood (G) based test of differentiation using the program GENEPOP on the Web (Raymond Rousset 1995; http://genepop.curtin.edu.au/). The overall significance will be determined via Fishers combined probability test. G-test analysis will be performed with collection points over the large scale with multiple collection points within the extensively sampled sites and finally among the colonies from each population to verify genetic differences will be used. Colonies will be classified as simple families when workers had genotypes consistent with being the direct offspring of one pair of reproductives and when the observed frequencies of the genotypes did not differ significantly from those expected under Mendelian segregation of alleles from two parents. Significance will be determines by a G-twst (P Colonies will be classified as extended families when the genotype distributions within colonies were not consistent with being produced by a single pair of reproductives (e.g more than four genotypes at a locus or three or more homozygote genotypes) or genotypes frequencies deviated significantly from those expected in simple families. Genetic relatedness among workers will be estimated for each colony and averaged over colonies for the same site will be using the computer program RELATEDNESS 5.00 (Queller Goodnight 1989) Inbreeding-adjusted estimate of relatedness r* that better reflects the number of reproductive presents in each colony by using Pamilo (1985) formula R*= r- [ 2FIT /(1+FIT)]/ [1-2FIT/(1+FIT)] I,C,T representing different colonies The breeding system and genetic differentiation among colonies will be investigated with hierarchial F statistics, assuming the infinite allele model and with individuals nested in colonies. The hierarchical analysis will be performed with F-stat program. It will be investigated whether there will be isolation by distance (a positive correlation between genetic differentiation and geographical distance) by calculating Fst between pairs of collection points within large colonies using the programe FSTAT 2.9.3.2 and by testing the significance of correlation between FST/(1-FST) and logarithm of geographical distances with MANTEL test (Mantel 1967). Computer program GENEPOP, with 10 000 permutations and the Mantel correlation coefficient r can be obtained with MANTEL (http://life.bio.sunysb.edu/morph/soft-mult.html) Estimating Colony Foraging Area Termite collected at a sampling point will considered to be affliated with a given colony if Fct, which measured genetic differentiation between collection points, will not significantly different from zero (i.e., 95% confidence interval did not overlap with zero. Fct will be measured between all possible combinations of collection points pair at each site. Variation in mitochondrial DNA markers will be provided additional support for colony designation (Bulmer et al. 2001). The maximum linear distance between collection points affiliated with the same colony will be used to estimate foraging range. For colonies that will be composed of three or more collection points (n+4), will be estimated the area encompassed by non intersecting lines drawn between collection points and adjusted so it did not overlap with neighboring collection points affiliated with different colonies. Swarming trap The swarm aggregation of alates will be collected alive with light traps. The traps consisted of buckets with a battery operated black light attached to attract the alates. Flight Observation Sticky trap will be used to monitor the dispersal flights of these species, which, in hindsight, may help explain some of the differences in how they are genetically structured. In addition their emergence and landing location. Sex ratio Sex ratio in swarm aggregations were tested for significant deviation from 1:1 using Binomial tests. Swarm aggregations were tested for significant genotypic differentiation with log likehood G Statistics using FSTAT 2.9.3.2 Bioassay Genetic mutation/change after being exposed to non repellent termiticide The concentrations were 0.001, 1.00, 10,100 and 1000 ppm for fipronil, imidacloprid and indoxacarb. In addition, distilled water was used as control for each termiticide. The method used for treatment was described by Hu (2005). Five kilograms of soil were collected from the site of the subterranean termite C. gestroi colony. The soil was then oven-dried. One kilogram for each termiticide was sieved and subsequently divided into six parts. The soil was placed in 100g increments into self-sealing plastic bags for treatment. Ten milliliters of the five concentrations of, fipronil, imidacloprid and indoxacarb will be added to the 100 g bag of soil for testing. The bags were sealed and allowed to mix thoroughly. The soil will be removed from the bags and placed in plastic bags (12 by 115 cm). The termiticide-treated soil was air-dried in a hood for ten days to allow solvents in the formulation to evaporate. Treatments were replicated three times using new stock solutions for each replicate. Bioassay design: Bioassay tubes designed by Su et al. (1995) will be used to investigate termite penetration in various thicknesses of termiticide-treated soil (Figure1). At time of testing, dried treated soil will be removed from the hood, placed into plastic bags and 10 ml of water was pipetted into the bags to obtain 10% moisture content. Three (3 cm) wooden applicator sticks were placed at the bottom of the glass test tube (2 by 15 cm), and 7% non-nutrient agar was inserted into the test tube until it rested on the wooden sticks. Oven-dried and sieved soil will be placed into plastic bags and moistened to concentrations of 10% (10ml /100g) to serve as untreated sand in the test designs. A 0.0, 1.0, 3.0, 5.0, or 10cm layer of termiticide-treated soil was centered between two layers of untreated soil (Figure.1). A glass funnel will be used to place the soil in the test tube and a scintillation vial (10ml) attached to the shank of a screwdriver was used to lightly and level the layers of soil. A 5 cm segment of termiticides-treated soil and a 5 cm segment of untreated soil served as control. Additionally, layers of untreated soil at various thicknesses in the bioassay tube will be served as control. The soil segment will be capped with a 1.0 cm core of agar and a 4cm by 4 cm portion of paper towel folded placed on the top of the tube. Eighty C. gestroi workers and five soldiers (20:1) were placed in each test tube to simulate normal termite activity and prevent overcrowding. A piece of aluminum foil will be placed over the top and end of each tube, and will be covered with black plastic container to allow for darkness and prevent desiccation of the termites. Bioassay tubes will be kept at 29 ±1 °C. The experiment was a 6 (concentrations) x 3 (replicates) x 5 (soil thickness) factorial design with concentration and thickness as the main effects. Test tubes will be held vertically in test tube racks with termites at the top of the tube. Observations will be made daily up to one week for the number of worker termite mortality, vertical distance of treated soil penetrated by termites and percentage of penetration into treated soil. The percentage of termite mortality and soil penetration data were subjected to arc-sine transformation before analysis of variance and means were separated with Tukey HSD. Then percentage of penetration into treated soil and the percentage of mortality were analyzed with analysis of variances (ANOVA). Also the percentage of surviving termites located at the bottom of the bioassay unit was analyzed. DNA will be isolated from dead termite to b

“The Other Side of Eden” by Brody

â€Å"The Other Side of Eden is directly based in Brody’s experience when he lived and leaned from Aboriginal Elders. Actually the book touches the question of American Aboriginal cultures and provides new anthropological perspectives to them. The author discusses the Canadian Aboriginal cultures such as Gitxsan, Dunne-za and Inuit. Brody challenges general anthropological assumptions concerning hunter-gathers societies. The book is divided into six chapters headed: language, creation, time, words, gods, and mind.The author tries to counter the idea that Aboriginal hunter-gatherers are not nomadic and provides evidence of their being agricultural culture. It is interesting to notice that Brody’s style of writing is highly figurative and has strong relation to his previous works on the same topic. The book is written from legal transcripts, from field notes and from the memory of the past years. The book is written for academy to honor the memory of lives shared by Brod y. (Brody 2000)It is necessary to mention that in his book the author employs new approach aimed at deconstructing the â€Å"hunter-gatherer trope†. Therefore the author collected and revisited field notes as well as historical documents. The legal transcripts are brought from the ethnographical link. It is known that Brody conducted original researches to find more evidence for the book. Brody calls for recognition respect of â€Å"hunter-gatherer societies† in his book as they preserve their land compared with the â€Å"agriculturalist transformations†. (Brody 2000)While many authors tend to limit the political associations suggested by ancestors of Aboriginal cultures in USA and to maintain the ecological integrity, Brody makes an attempt to overcome those limits. It is apparent that he is not interested in natural ecology, because he strongly deconstructs the myth tat â€Å"indigenous systems that existed before contact were maladaptive to their various env ironments†. He provides the evidence that those Aboriginal cultures are unable to successfully adapt to economic development as well as natural resource development – they wouldn’t understand why and how to use oil and gas, for example.Nevertheless, the book has some negative moments. For example, Brody appeared to be unsuccessful in explaining nomadic and sedentary economic systems. Therefore it is possible to suggest that the author demonstrate a certain limit in his researching and writing from â€Å"outside one's own culture†. (Brody 2000) The author prefers to use the first person in his telling as if he is sitting nearby and leads the original conversation. However, the author is invisible hand in the book and his narrative moves readers further.It is noted that â€Å"the Other Side of Eden† is likely to be work-narrative or travel-narrative of his life and of interfaces with Aboriginal Elders. The author definitely challenges modern ideas and provides evidence that Aboriginal peoples weren’t historically wasters, because according to author â€Å"nothing is wasted†. The author convincingly demonstrates that problems of modern environment don’t result from actions of sedentary â€Å"hunter-gatherers societies†.The reason of those problems is globalization of agriculturalism which is considered nomadic and destructs environmental processes. (Brody 2000) In conclusion it is necessary to provide personal evaluation of the book. It is necessary to note that â€Å"The Other Side of Eden† is a real challenge to anthropological classification of so-called â€Å"hunter-gatherer†. The writing of the author seems authoritative, though Brody is respected by academics. In principle the book is worth for scholars and critics as well as for students and artists.Brody’s evidence provided in the book has strong and weak point. The strength of the boo is that author is not afraid of implem enting new assumptions and ideas and he is ready to counter existed stereotypes, whereas the weak moment is that author appears to be unable to provide proper explanation of some economic systems. Therefore the book undergoes both approval and critique. (Brody 2000) References Brody, H. (2000). The Other Side of Eden: Hunters, Farmers, and the Shaping of the World. Vancouver: Douglas & McIntyre.

Swot Poland

SWOT analysis for wine in Poland This module assists exporters and BSOs in developing countries in becoming familiar with the strengths, weaknesses, opportunities and threats for a wine exporter planning to target the Polish market. It also helps DC exporters to make a SWOT analysis, specific to their company. A SWOT (Strength, Weaknesses, Opportunities and Threats) analysis helps you to audit your company in relation to market developments and competitors on selected markets.The SWOT shown below visualises external aspects (opportunities and threats) on the Polish market and internal aspects (strengths and weaknesses) of your company, both affecting your market entry. You are only in control of internal elements. You can adapt and improve their intrinsic quality according to market trends, and the resulting opportunities and threats, in your target markets. In Figure 1 you can find a SWOT analysis for a DC exporter of wine to Poland. Note that this analysis is generalised, and shoul d be used only as an example.The CBI Export Marketing Planner (available at http://www. cbi. eu) offers tools to help you make the SWOT analysis specific to your company and your target market. Firstly, the 5Ms methodology helps you audit your company on the basis of five variables; Men (human resources), Means (resources), Methods (management and process performance), Machines, and Measurables (marketing impact, customer and employee satisfaction, qualitative certifications). Secondly, you can apply the value chain approach to analyse all value-addition activities of your company.Identifying external factors is done through a market audit. The character of all these elements is that they are beyond your control. You cannot influence them. The market dictates the rules of the global play you are about to enter. The key question here is: can you deal with the market trends? Do the trends offer you chances or challenges for export success? Together with the competitor analysis, it wil l determine your decision on final market selection and market entry strategy. CBI modules on your product (group) offer a lot of information on market developments, threats and opportunities.The third tool offered by the Export Marketing Planner is the STEEP methodology. This is a comprehensive method for identifying opportunities and threats related to Socio-cultural factors, Technological factors (barriers), Economic factors, Environmental factors and Political factors related to trade. Source: CBI Market Information Database †¢ URL: www. cbi. eu †¢ Contact: [email  protected] eu †¢ www. cbi. eu/disclaimer SWOT analysis for wine in Poland Figure 1 SWOT analysis for wine to the Poland SWOT: DC EXPORTER OF WINE TO POLAND STRENGTHS †¢ †¢ †¢ †¢ †¢ †¢Availability of cheap land and raw materials Favourable climate Larger production volume Innovative character of wine compared to Old World wines Competitive price Availability of low-cost hu man resources Unique grape varieties †¢ †¢ WEAKNESSES Transportation costs Lack of (access to) capital (investment/working) and export insurance Lack of access to market intelligence Lack of export/management experience Lack of Quality Assurance Systems Lack of entrepreneurial capacity Lack of tacit knowledge †¢ †¢ †¢ †¢ †¢ OPPORTUNITIES †¢ †¢ †¢ †¢ A new wine market with expanding possibilities and un-established tastes Expanding market share of table wine The domination of cheap wine can make entry for some DC exporters easier Low import tariffs Emergence of small independent importers dealing with small volumes of wine Increased capacity for bulk imports (for producers of large quantities) Local producer networks; sharing of knowledge †¢ THREATS Dominance of semi-dry and sweet wines can limit distributors focusing on dryer varietiesHigh volume requirements by importing hypermarkets, which lay a dominant role in Poland DC s face competition from each other Cultural and language differences EU quality assurance and legislative requirements difficult for DC producers (e. g. adding sugar) Trust in Eastern European brands due to tradition Preference for cheap table wine as a threat to market integration of more premium wines †¢ †¢ †¢ †¢ †¢ †¢ †¢ †¢ †¢ This survey was compiled for CBI by ProFound – Advisers In Development in cooperation with Theo Jansen Disclaimer CBI market information tools: http://www. cbi. eu/disclaimer Source: CBI Market Information Database †¢ URL: www. cbi. eu †¢ Contact: [email  protected] eu †¢ www. cbi. eu/disclaimer

Traditional Chinese Medicine And Modern Medicine - 2021 Words

Traditional Chinese medicine, or TCM, is one of the oldest systems of medicine to exist among humans, and it is extremely prominent to this day. According to legend, the system has been in use since the third millennium BCE. The views of such an old system differ greatly from those found in modern western medicine. Perhaps most prominent is the fact that TCM focuses more on the patient than the illness. The system considers the human to be a microcosm of the universe, implying that the body is affected by the traditional Taoist forces of yin and yang, the five elements, and Qi in the same way the cosmos are. Each of these forces act upon the â€Å"zang fu,† or organ system, directly influencing a patient’s health. Healers use qigong to assist in the restoration of stability in the body. Achieving balance, both internally and externally, is the most important aspect of healing in TCM. The traditional Indian medicine system, or TIM, is a system focused entirely on self-healing or â€Å"Ayurveda.† Like TCM, TIM is a very ancient system, dating back at least four thousand years ago, and is very different from western medicine. TIM follows the ancient Sankhya philosophy that the universe is One Self, it is inside everyone and everyone is a part of it – this belief is also known as Brahmanism. Two large aspects of TIM are yoga and Ayurveda. Yoga teaches patients to move away from their outer body and identity toward their inner Self inside their hearts. Ayurveda is the act of healing byShow MoreRelatedTraditional Chinese Medicine Essay1141 Words   |  5 PagesOne important aspect of Chinese culture is Traditional Chinese Medicine, also known as TCM. Chinese medicine has been around for quite awhile, and is still around today. In the United States, we see it as acupuncture and massage. TCM is still widely popular in its home country where it is still pra cticed as it was a few centuries ago. Chinese medicine is evolving to our modern day times, but it is keeping close ties to its roots. Chinese medicine is also becoming more affluent in different partsRead MorePhilosophy And Ideology Of Chinese Medicine1723 Words   |  7 PagesChinese Medicine after modification the essay title is as follows Philosophy and Ideology of Chinese Medicine He who takes medicine and neglects to diet wastes the skill of his doctors Medicine can only cure curable disease, and then not always (Chinese Proverb) Theology: In China, one can observe aninterestingcombination of modern and traditional medical practices. There are some delusions about Chinese Medicine. Although the main philosophies and techniques are thousands of years old, theRead MoreAncient Chinese medicine, originating from almost 2,000 years ago, is a set of traditions that has1700 Words   |  7 PagesAncient Chinese medicine, originating from almost 2,000 years ago, is a set of traditions that has influenced modern medicine because of its diverse methods, techniques, and discoveries. Since its beginning, it has yet to diverge from its original practices: â€Å"A visit to a traditional Chinese pharmacy is like a visit to a small natural history museum. The hundreds of cabinet drawers, glass cases, and jars in a typical pharmacy hold an enormous variety of desiccated plant and animal material:Read MoreThe Effects Of Malaria On The Worlds Poorest1472 Words   |  6 Pagesblood cells along with causing complications with the kidneys, lungs, and brain. In more serious cases, it can cause permanent neurological effects and even death. 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For example, â€Å"Qi and blood deficiency or qi stagnancy aggravated by blood stasis may cause the retardation of the menstrual flow and result in dysmenorrhea.† (A World of Chinese Medicine) However, it can be classified as hot or cold; excess or deficiency; or blood or qi stagnation. (yin and yangRead MoreChinese Medicine And China History And Eastern Culture929 Words   |  4 PagesThe Chinese medicine is related to china history and eastern culture. Every culture has its own idea about creation of universe and human being. Chinese has a basic theory for human creation that included the human’s physiology and the human’s substance. The theory of five substances depends on five elements that related to all physiologic activity of human. One of them is Qi. Qi term has a conflicted idea between traditional Chinese medicine and western scientific sense that due to misunderstandRead MoreParasitic Diseases Affecting The Worlds Poorest1467 Words   |  6 Pagesblood cells along with causing complications with the kidneys, lungs, and brain. In more serious cases, it can cause permanent neurological effects and even death. As the Nobel Assembly said at the anno uncement of the Nobel Prize in Physiology or Medicine, â€Å"Diseases caused by parasites have plagued humankind for a millennium and constitute a major global health problem. In particular, parasitic diseases affect the world’s poorest populations and represent a huge barrier to improving human health andRead MoreMulticultural Assignment : Han Chinese Culture1094 Words   |  5 PagesMulticultural Assignment Han Chinese is the largest ethnic group not only in China but also worldwide. It is estimated that 1.16 billion people are of Han nationality. Therefore, this means that Han Chinese make up 20% of the world’s population (NCCIH, 2013). As a result, Han Chinese culture has had a significant impact throughout the world and their culture has spread throughout the world particularly in Taiwan, Thailand, Malaysia, North America and Europe. The Han Chinese people rely predominately

Racism At The Institute On Race And Poverty - 1344 Words

Jordan Reber Mr. Bergmann Senior English P3 28 August 2015 Racism in America According to the Institute on Race and Poverty (IRP) in 2000 a white individual making the same income annually as any other race has a seventy-eight percent chance of owning a home, and only a twenty-two percent chance of having their credit denied on a loan, however, minorities like Blacks and Hispanics have a significantly lower chance of both. (Lawrence, and Keleher 3). In fact, Blacks with those same specifications only have a forty-eight percent chance to own a home, and an astounding forty-five percent chance to have their credit denied on a loan. Meanwhile, Hispanics have a forty-six percent chance to own a home, and a thirty-percent percent chance of having their credit denied. While this may seem overtly discriminatory with just a glance, one must first delve into what those numbers actually mean, and the details went into creating those numbers, as well as take a peek into the institution that created those numbers before jumping to the conclusion that it is empirically racist. Recently, racism has exploded into the spotlight worldwide, and especially in the United States of America with stories of racially motivated police brutality and a supposed â€Å"race war† taking center stage. All this coming forty-seven years after Dr. Martin Luther King Jr. sought to end racial tension and racist governmentShow MoreRelatedRace Class Gender 456 Final Exam1311 Words   |  6 PagesL.ADKINS RCG 456 FINAL EXAM L. Adkins Race Class Gender 456 Final Exam Laura Adkins DSU Student â€Æ' L. Adkins Race Class Gender 456 Final Exam 1. Elaborate Noel’s theory on the contact situation of race relations (20 pts). 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My Letter The Investigative Personality - 967 Words

My three letter RIASEC code is IRC. The first letter represents the investigative personality. My preferred work environment as an investigative person is in a fairly unstructured environment where I can be left alone to explore problems that attract my curiosity. My management style is very lenient, I would like to present my problems to my employees and give them the freedom to follow through. Some of the suggested occupations that interest me are biologist, psychologist, and dental hygienist. The second letter of my RIASEC code stands for the realistic personality. My preferred work environment as a realistic person is in a place where I can tackle concrete problems and obtain tangible results and do not hate to engage in a lot of social interaction. The management style of a realistic type is preferring to solve a problem myself instead of spending time to explain it to someone else. One of the suggested occupations for a realistic person that I might enjoy is an occupational the rapist. The final letter of my RIASEC code stands for the conventional personality. My preferred work environment as a conventional person would be being a part of a large organization. My management style is very relaxed because I do not enjoy leadership positions. A few conventional careers that interest me are secretary, banker, and dental assistant. My reported personality type according to the results of the Myers-Briggs Type indicator is ISTP. 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If an individual’s personality has characteristics of a certain trait, such as creativity or idealism, it would be a good idea to get into a career that emphasizes this trait and maximizes that potential. In career counseling there are many theories that are acceptable for utilization in client-counselor relationships, but one in

A Woman of No Importance, Final Act free essay sample

Wilde uses many dramatic effects throughout the play to shock and amuse the audience and many of them can be seen in this final scene. The fact that this conversation between Mrs Arbuthnot and Lord Illingworth takes place in Mrs Arbuthnot’s house, her personal space and territory puts her at an advantage and it shows that Lord Illingworth is surrendering his usual control over his situations By Lord Illingworth referring to Mrs Arbuthnot as ‘Rachel’ we are again made aware that we are listening to two people who have a strong past relationship. She calls him ‘George Harford’ while he uses her name far less often that in the persuasive Act 2. During this scene, Lord Illingworth speaks with awareness of the legal situation, he knows he can never make Gerald legitimate but he is willing to leave him property â€Å"What more can a gentleman desire in this world? † and Mrs Arbuthnot’s response of â€Å"Nothing more, I am quite sure† turns this in to a class confrontation. When Mrs Arbuthnot says â€Å"I told you I was not interested, and I beg you to go. † this is a threat to conventional society and the audience would have been shocked by this. She treats Lord Illingworth as he once treated her, in purely financial terms and she tells him that Gerald no longer needs his money, â€Å"You come too late. My son has no need of you. You are not necessary. † She then goes on to explain to him that Gerald and Hester are in love and they don’t need his money because Hester already has money of her own. Lord Illingworth asks where they will go and Mrs Arbuthnot’s reply â€Å"We will not tell you, and if you find us we will not know you. You seem surprised. What welcome would you get from the girl whose lips you tried to soil, from the boy whose life you have shamed, from the mother who dishonor comes from you? † is very melodramatic and it also relives the fact that Lord Illingworth tried to kiss Hester and this is when Gerald found out that he was his father, â€Å"Lord Illingworth you have insulted the purest thing on Gods earth†. This leaves Lord Illingworth to admit that he wants Gerald, â€Å"Rachel, I want my son. † Wilde uses many props in this scene, the main one being the letter Gerald has written to Lord Illingworth imploring him to marry his mother. The audience know what is written in the letter before Lord Illingworth does and this adds drama and tension because the audience are waiting for the big reveal and to see what happens. This letter also links back to the letter that Lord Illingworth sees in Act 2 and says â€Å"What a curious handwriting! It reminds me of the handwriting of a woman I used to know years ago. † and his dismissal of it so simply. The stage direction of ‘Mrs Arbuthnot watches him all the time’ is very important because she wants to see his reaction. Ironically his proposal of marriage after reading Gerald’s letter uses similar language to Mrs Arbuthnot’s when explaining to Gerald why she would refuse him, for her marriage would be a ‘sacrifice’ and for Lord Illingworth it would be a ‘surrender’. For Mrs Arbuthnot to say this at this point in the play would have been very uncommon for the time because the audience would be expecting a happy ending, for the fallen women to marry the father of her child or for it to end like a melodrama, in tragedy. For the first time, Mrs Arbuthnot is triumphant against Lord Illingworth with the repetition of his own words when she says, â€Å"Children begin by loving their parents. After a time they judge them. Rarely if ever do they forgive them. † Lord Illingworth is clearly surprised at this response and then resorts to cruelty. His parting speech creates an exciting climax as the censorship of the time wouldn’t allow anyone to say the word ‘bastard’ on the stage. Wilde’s stage direction of Mrs Arbuthnot’s use of the glove â€Å"Mrs Arbuthnot snatches up glove and strikes Lord Illingworth across the face with it† is a very good use of a prop because in the time this play was written a glove was a very masculine item and being hit with one was a sign of violence and confrontation. The audience is allowed a shock, due to the word about to be spoken and then they get a relief as the taboo is maintained by Mrs Arbuthnot cutting Lord Illingworth off before he can finish his sentence because she will not let him say the word because she doesn’t want to hear him say this about her beloved son. The villain is punished and Mrs Arbuthnot’s respectability is ma intained. All of this is typical of a melodrama and we the audience now feel something has been accomplished. Wilde’s use of stage directions are very well placed and are very dramatic, especially the last few lines of this scene when Mrs Arbuthnot ‘falls sobbing on the sofa’ and it reinforces that this play is a melodrama because people are not usually this dramatic in normal everyday life. Gerald and Hester now return to Mrs Arbuthnot and we have the image of ‘a man and a woman in a garden’ which has been mentioned previously throughout the play and is a sign of sex and fertility and in this scene it shows the audience the image of a new family emerging. Due to Hester having changed her views from believing that women who have children outside of the laws of marriage should be punished, â€Å"A woman who has sinned should be punished, shouldn’t she? † And that the children should also carry this shame, â€Å"Yes, it is right that the sins of the parents should be visited on the children. It is a just law. It is God’s law. † to her now saying â€Å"I was wrong. Gods law is only love. † Because she is in love with Gerald and has managed to listen and understand all of the things that Mrs Arbuthnot has had to face to bring up Gerald alone. At the end of the play when Gerald sees the glove lying on the floor Mrs Arbuthnot picks up and changes the title line of the play and once again mirrors Lord Illingworth’s statement about seeing the letter from Mrs Arbuthnot, â€Å"Oh! o one. No one in particular. A Man of no importance. † Unmarried and defiant she enters into a fresh and better world although the 19th century attitudes to marriage are still upheld in a way because even though she has won against Lord Illingworth and she has managed to keep Gerald and now has the love and respect of Hester the audience are still left with the image of them being exiled to America, where they have less strict views on illegitimacy and have more freedo m.